ABSTRACT
Objective To investigate the effects of PRPF19 knockdown on the proliferation, migration, and invasion of pancreatic cancer cells. Methods The expression of PRPF19 in pancreatic cancer and normal tissues was analyzed using the GEPIA database. The protein and mRNA expression levels of PRPF19 in pancreatic cancer cells were detected by Western blot and qRT-PCR. Small interfering RNA (siRNA) was used to silence the expression of PRPF19 in pancreatic cancer cells, and the knockdown efficiency was verified by Western blot and qRT-PCR. CCK-8, colony forming, and Transwell assay were used to detect the effects of knockdown of PRPF19 on the proliferation, colony forming, migration, and invasion of pancreatic cancer cells. Results GEPIA analysis showed that PRPF19 was highly expressed in pancreatic cancer tissues compared with normal pancreatic tissues. In comparison with normal pancreatic cells, PRPF19 was highly expressed in various pancreatic cancer cell lines such as MIA PaCa-2 and PANC-1 (P < 0.05). In comparison with the control group, PRPF19 knockdown significantly reduced the proliferation rate, colony forming, cell migration, and invasion of pancreatic cancer cells (P < 0.05). Conclusion PRPF19 knockdown inhibits the proliferation, migration, and invasion of pancreatic cancer cells. PRPF19 may play an important role as an oncogene of pancreatic cancer.
ABSTRACT
Objective To investigate one -step overall treatment to improve the condition of diabetic foot ulcers based on transluminal technology.Methods The patients with diabetic foot ulcers were treated with percutaneous transluminal angioplasty(PTA)to open blood flow,intensively control patients'blood glucose,application of sensitive antibiotics,erode debridement and dressing change according to wound bed preparation(WBP)and moist wound heal, vacuum aspiration was applied in large wound.Results Eighty -two patients(eighty -five lower limbs)underwent PTA,the successful rate was 100.0%.Of 116 ulcers,106 cases were effective (91.4%),10 cases were invalid (8.6%).Their ankle -brachial index (ABI)increased from preoperatively (0.41 ±0.12)to postoperatively (0.75 ± 0.09).Transcutaneous oxygen pressure(TcPaO2 )increased from preoperatively (14.6 ±4.8)mmHg to postopera-tively (51.6 ±8.9)mmHg.Conclusion One -step treatment regarding multi disciplinary team on endocrine section can improve the treatment of diabetic foot and lower the amputation rate.